In this book, four new sections have been added in this edition containing multiple choice questions (MCQs), problem oriented case studies, very short question with answer and Biochemistry course curriculum of different Universities. MCQ will definitely be useful to the students appearing for PG entrance examinations later. Problems relating to biochemical case studies have recently been introduced in question papers of many medical universities and in this context, these will be of immense help to the students for diagnostics analysis. In this new edition very recent questions have been incorporated, some short questions with answers and new case history are added. Guide line of Medical Council of India (MCI) for Biochemistry course curriculum is appended, so the students will be aware about the must know and desirable to know area in the subject. Through separate chapters have been earmarked for paper I & II in the courses of study of different Universities, sometimes the question setters do not strictly adhere to the instructions laid down in it, while setting questions. Although it happens occasionally the students have to bear this anomaly as there is no remedy for it.